The DP World Tour has issued a £100,000 fine to players who competed in June’s Saudi Arabian-backed LIV Golf event and banned them from the Scottish Open.
The players can also not play in the PGA Tour’s Barbasol Championship and Barracuda Championship in July.
The Scottish Open is one of the DP World Tour’s most lucrative events and is used by many players as preparation for The Open, held the following week.
The PGA Tour suspended 17 members who played in the inaugural LIV Golf event.
Players who have joined the LIV Golf series are allowed to play in the 150th Open Championship at St Andrews.
The DP World Tour, formerly known as the European Tour, has also warned players that “participation in a further conflicting tournament or tournaments without the required release may incur further sanctions”.
The next LIV Golf event in the $250m (£200m) eight-event invitational series is scheduled to take place in Portland, Oregon from 30 June to 2 July.
South Africa’s Charl Schwartzel claimed the first LIV Golf event at Centurion Club in Hertfordshire, winning $4.75m (£3.86m).
Lee Westwood, Ian Poulter, Sergio Garcia, Martin Kaymer and Graeme McDowell are the highest-profile DP World players who will be affected by these sanctions.
Keith Pelley, DP World Tour chief executive, said: “Every action anyone takes in life comes with a consequence and it is no different in professional sport, especially if a person chooses to break the rules. That is what has occurred here with several of our members.
“Many members I have spoken to in recent weeks expressed the viewpoint that those who have chosen this route have not only disrespected them and our Tour, but also the meritocratic ecosystem of professional golf.
“Their actions are not fair to the majority of our membership and undermine the Tour, which is why we are taking the action we have announced today.”
Four-time major champion Brooks Koepka became the latest player to join LIV Golf earlier this week.